Rapid differentiation of human pluripotent stem cells into …

Nervous system development and neural cell differentiation have been studied extensively, providing a foundation for current protocols of in vitro generation of …

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Rapid detection and differentiation of dengue virus …

These reported methods for detection and differentiation of LAMP results were done by real time monitoring of turbidity (at 400 nm) with a Loopamp real-time turbidimeter, "ladder-like feature" on agarose gel electrophoresis and color change from orange to green caused by fluorescent detection reagent. However real time monitoring …

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Rapid and accurate identification of stem cell differentiation …

The differentiation experiment was replicated three times, and for each cell state, spectra were collected from at least 20 cells. 10 SERS spectra were obtained for each cell by selecting random locations, encompassing both the central and peripheral regions of each cell. The spectral data can be divided into 12 categories, labeled as …

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Rapid differentiation of Lactobacillus species via metabolic …

Rapid differentiation of Lactobacillus species via metabolic profiling J Microbiol Methods. 2018 Nov:154:147-155. doi: 10.1016/j.mimet.2018.10.013. Epub 2018 Oct 23. Authors Kundi Yang 1, Mengyang Xu 1, Fanyi Zhong 1, Jiangjiang Zhu 2 Affiliations 1 Department of Chemistry and ...

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Rapid differentiation of hiPSCs into functional …

Transcription factors (TFs) have been introduced to drive the highly efficient differentiation of human-induced pluripotent stem cells (hiPSCs) into lineage-specific oligodendrocytes (OLs). However, effective strategies currently rely mainly on genome-integrating viruses. Here we show that a synthet …

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differentiation of rappid

differentiation of rappid Home Page Back In this study, direct ionization mass spectrometry (DI-MS) has been developed for rapid differentiation of Ganoderma (known as Lingzhi in Chinese), a very popular and ...

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Derivative Calculator

Advanced Math Solutions – Derivative Calculator, Implicit Differentiation. We've covered methods and rules to differentiate functions of the form y=f(x), where y is explicitly defined as... Enter a problem. Cooking Calculators. Cooking Measurement Converter Cooking Ingredient Converter Cake Pan Converter More calculators.

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Rapid differentiation of hiPSCs into functional

Transcription factors (TFs) have been introduced to drive the highly efficient differentiation of human-induced pluripotent stem cells (hiPSCs) into lineage-specific oligodendrocytes (OLs).

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Rapid differentiation of tea products by surface desorption …

Protonated water molecules generated by an ambient corona discharge were directed to impact tea leaves for desorption/ionization at atmospheric pressure. Thus, a novel method based on surface desorption chemical ionization mass spectrometry (DAPCI-MS) has been developed for rapid analysis of tea pro …

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Rapid and high-purity differentiation of human medium …

Traditional methods of neural differentiation, such as induction and picking of neural rosettes and multi-step chronological addition of cytokines have resulted in the generation of various neural subtypes, including MSNs, but with high variability or excessive duration of differentiation [18–23].

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Rapid and Efficient Directed Differentiation of Human …

Controlling the differentiation of human pluripotent stem cells is the goal of many laboratories, both to study normal human development and to generate cells for transplantation. One important cell type under investigation is the retinal pigmented epithelium (RPE). Age-related macular degeneration (AMD), the leading cause of …

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Rapid and Reproducible Differentiation of Hematopoietic and …

The differentiation of hPSCs takes place in two steps: first the induction of hematopoietic stem/progenitor cells (HSPCs), then the induction of lymphopoiesis by Notch signaling. However, the differentiation of T cells from hPSCs can be difficult and lack reproducibility. One parameter that needs to be better assessed is the potential of DLL1 ...

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Rapid differentiation of phenotypically similar yeast …

Single-strand conformation polymorphism (SSCP) analysis of ribosomal DNA (rDNA) was investigated for rapid differentiation of phenotypically similar yeast species. Sensitive tests indicated that some yeast strains with one, most strains with two, and all strains with three or more nucleotide differe …

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Efficient differentiation of human embryonic stem cells to …

Differentiation was initiated as described above with slight modifications, using combinations of SB, LDN, CKI, and NIC. Aggregates (~ 1200 cells/body) were formed at day 2 and cultured in suspension until day 6 in their respective primary differentiation conditions. At day 6, aggregates were plated on Growth Factor-Reduced Matrigel (GFR …

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Rapid differentiation of Chinese hop varieties (Humulus

Rapid differentiation of Chinese hop varieties (Humulus lupulus) using volatile finger by HS-SPME-GC-MS combined with multivariate statistical analysis J Sci Food Agric. 2018 Aug;98(10):3758-3766. doi: 10.1002/jsfa.8889. Epub 2018 Mar 2. Authors Zechang Liu 1 ...

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Rapid Differentiation of Aspergillus Species from Other …

We developed a PCR-based assay to differentiate medically important species of Aspergillus from one another and from other opportunistic molds and yeasts by employing universal, fungus-specific primers and DNA probes in an enzyme immunoassay format (PCR-EIA). Oligonucleotide probes, directed to the internal transcribed spacer 2 region …

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Rapid differentiation of Staphylococcus aureus in blood …

Rapid and accurate identification of Staphylococcus aureus (SA) in blood culture specimens is crucial for timely clinical intervention. Traditional Gram staining methods, although widely accessible and cost-effective, exhibit variable sensitivities and specificities in the BACTEC system. We a …

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Rapid differentiation of infectious salmon anemia virus …

Infectious salmon anemia virus (ISAV; Isavirus salaris) causes an economically important disease of Atlantic salmon (Salmo salar L.). ISA outbreaks have resulted in significant losses of farmed salmon globally, often with a sudden onset. However, 2 phenotypically distinct variants of ISAV exist, each with divergent disease outcomes, associated …

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Rapid Differentiation of Phenotypically Similar Yeast …

Differentiation of closely related basidiomycetous yeast species. In basidiomycetous yeasts, the Bullera mrakii clade contains 11 closely related species which are also phenotypically similar . The lengths of the amplified fragments covering the whole ITS1 region from all 11 species except Bullera hubeiensis were 258 to 281 bp.

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Development of a rapid screen for the endodermal differentiation …

A challenge facing the human pluripotent stem cell (hPSC) field is the variability observed in differentiation potential of hPSCs. Variability can lead to time consuming and costly optimisation to ...

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Rapid differentiation of human pluripotent stem cells into …

Nervous system development and neural cell differentiation have been studied extensively, providing a foundation for current protocols of in vitro generation of various neuronal cell types from pluripotent stem cells and somatic cells 1,2.Current neuronal cell differentiation protocols go step-by-step through a series of stages in …

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Rapid and efficient differentiation of functional motor …

In this study, we present a simple, rapid protocol for differentiation of human iPSCs into functional spinal (lower) MNs, involving only adherent culture and use of small molecules for directed differentiation, with the ultimate aim of rapid production of electrophysiologically functional cells for short-term neural injury experiments. We show ...

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Rapid and Reproducible Differentiation of Hematopoietic …

In this article, we describe an efficient, rapid and reproducible protocol for the generation of hematopoietic and T cell progenitors in two steps: (1) generation of …

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Different Classes Of Rapids Explained – The International …

Even the nastiest, most aggressive whitewater rapids can be broken down to the same staple river features and the four basic elements. When you look at a rapid and break it down in your head, you'll find the same four parts – either separate or in a combination – because they are what creates these rapids in the first place. …

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Rapid differentiation of hiPSCs into functional …

Introduction. Oligodendrocytes (OLs), which are myelinating cells of the central nervous system (CNS), are extremely promising therapeutic targets for cell replacement-based therapies for myelin loss or dysfunction, such as in patients with multiple sclerosis and white matter ischemic injury 1–4.Reprogramming human-induced …

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Driving Mechanism of Differentiation in Urban …

To achieve sustainable urban development, it is essential to gain insight into the spatial and temporal differentiation characteristics and the driving mechanisms of the urban thermal environment (UTE). As …

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Rapid Neuronal Differentiation of Induced Pluripotent Stem …

Neurons derived from human induced Pluripotent Stem Cells (hiPSCs) provide a promising new tool for studying neurological disorders. In the past decade, many protocols for differentiating hiPSCs into neurons have been developed. However, these protocols are often slow with high variability, low repr …

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Soluble Amyloid Precursor Protein Induces Rapid Neural Differentiation …

To further investigate whether increased sAPP secretion plays a role in the neural differentiation of APP hESCs, we treated control H9 hESC cultures with recombinant sAPPα or sAPPβ protein. 0 n m, 1 n m, 10 n m, or 100 n m of each recombinant protein was added to H9 cells growing in mTeSR® media, and protein …

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Microfabric Vessels for Embryoid Body Formation and Rapid

The period of NSC differentiation was significantly shortened under high EB density culture conditions. Simultaneous mass production of a pure population of NSCs was possible within 4 days. These ...

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